Abstract
In this protocol, L1 stage larvae are collected that carry nuclear-localized GFP/wCherry reporters. These can be fixed so that the GFP/wCherry maintains nuclear localization and stain nuclei by DAPI. This protocol therefore achieves the collection and fixation of nuclei in worm L1 larvae.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol has been adapted from Rigaut et al. (1999) and Puig et al. (2001).
References
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