Abstract
Virus overlay assay is a method to detect protein-protein interaction in vitro. We performed the virus overlay assay to identify the receptor proteins interacting with the infectious spleen and kidney necrosis virus (ISKNV).
Keywords: Far-Western blotting, Protein, Virus
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Kikkert et al. (1998).
References
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