Abstract
SVZ-derived progenitors grown as neurospheres is a well-known model to study neural stem cell and progenitor functions such as proliferation, differentiation/self-renewal, and migration (Durbec and Rougon, 2001). This protocol is for preparing a culture of SVZ-derived progenitors from 8 early postnatal mouse brains (P0 to P3). One week after cell plating, we can observe round floating neurospheres, each resulting from the clonal expansion of a single EGF/FGF responsive neural progenitor.
Keywords: Nervous system, Adult brain, Stem cells, Subventricular zone, Primary culture
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Durbec and Rougon (2001) and Vernerey et al. (2013).
References
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