Abstract
Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This protocol was used to map genes (Sharakhova et al., 2010) and microsatellite markers (Kamali et al., 2011; Peery et al., 2011) on polytene chromosomes from ovarian nurse cells and salivary glands of malaria mosquitoes. Detailed physical genome mapping performed on polytene chromosomes has the potential to link DNA sequences to specific chromosomal structures such as heterochromatin (Sharakhova et al., 2010). This method also allows comparative cytogenetic studies (Sharakhova et al., 2011; Xia et al., 2010), and reconstruction of species phylogenies (Kamali et al., 2012).
Keywords: Mosquito, Chromosome, Mapping, Genome, FISH
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The protocol was adapted from previously published papers: Sharakhova et al. (2010) and Kamali et al. (2012). We thank the Chinese Centre for Disease Control and Prevention, Shanghai, China for providing an Anopheles sinensis colony. This work was supported by a National Natural Science Foundation of China (31301877) to AX and by a National Institutes of Health (Bethesda, MD, U.S.A.) grant (5R21AI094289) to IVS. AP and IVS were supported in part by the Institute for Critical Technology and Applied Science (ICTAS) and the NSF award 0850198.
References
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