Abstract
Plants have developed two distinct mechanisms, i.e., strategy I (reduction strategy) and II (chelation strategy), to mobilize insoluble Fe(III) in the rhizosphere and transport it through the plasma membrane. Arabidopsis thaliana and other dicots rely on strategy I. In this strategy, the rhizosphere is first acidified by a PM-localized H+-ATPase, AHA2. Then, FERRIC CHELATE REDUCTASE 2 (FRO2) reduces Fe(III) to soluble Fe(II). Finally, the reduced Fe is taken up by a high-affinity transporter, IRON-REGULATED TRANSPORTER 1 (IRT1). Root ferric chelate reductase activity can be quantified spectrophotometrically by the formation of Purple-colored Fe(II)-ferrozine complex in darkness.
Keywords: Arabidopsis thaliana, Ferric chelate reductase, FRO, Enzyme activity, Root
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Yi and Guerinot (1996).
References
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Shoots are separated from the roots by sharp scissors before taking the fresh weight. Make sure your assay solution is ready waiting in the tubes before you do all cutting and transfer of roots so that the roots do not dry out.
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