Abstract
This protocol shows how to characterize the dynamics of hippocampal neurogenesis in the adult mouse by describing preparation of brain tissue, immunofluorescence of brain sections and confocal stereotactic cell counting.
Materials and Reagents
Equipment
Software
Procedure
Representative data
Not all Tbr2 Eomes antibody (AB) lots work for this protocol. Below are representative pictures and sample lots that show a specific staining (A) of Tbr2 in vibratome sections and antibody lots that give only unspecific background staining (B). Red arrows in A show Tbr2 staining. The green arrows indicate unspecific background staining of glia-like cells that might appear in the specific antibody lots. This background staining is however very well distinguishable from the nuclear Tbr2 staining. Figure 2. A specific staining (A) of Tbr2 in vibratome sections and antibody lots that give only unspecific background staining (B).
Recipes
Acknowledgments
This protocol is adapted from Seib et al. (2012).
References
Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol. You are highly recommended to post your data including images for the troubleshooting.
You are highly recommended to post your data including images for the troubleshooting.