Abstract
Superoxide dismutase (SOD) acts as a primary defence against reactive oxygen species (ROS) by converting O2- to O2 and H2O2. Members of this enzyme family include CuZnSOD, MnSOD and FeSOD. Most eukaryotes harbor CuZnSOD and MnSOD, and FeSOD is found in plants and prokaryotes. This protocol is to demonstrate how to prepare the cellular extract for the identification and characterization of SODs in planta.
Keywords: SOD, Activity Assay, N
Materials and Reagents
Equipment
Procedure
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References
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You only need is the SDS eliminated 12.5% PAGE (without SDS), which is good for in gel SOD activity assay, best,
Hi,Please extract your sample with ice-cold buffer, and then run your gel in cold room,Which can give you best result,Sure, SOD may or may not denature when temp higher then 4C, different SOD from different sample do have it optimal reaction temp and pH value,Best,