Abstract
Availability of ion specific fluorescent dyes has enabled the possibility to perform in vivo ion specific measurements using live cell imaging in many cellular compartments (Krebs et al., 2010; Bassil et al., 2011; Halperin and Lynch, 2003; Swanson et al., 2011; O'Connor and Silver, 2007). The importance of ion and pH homeostasis of intracellular compartments, including the vacuole, to cell growth is critical and well established (Krebs et al., 2010; Bassil et al., 2011).* Dedicated to Stephen Halperin who tragically and unexpectedly passed away.
Keywords: Plant nutrition, PH, Ion transport, Homeostasis, Live cell imaging
Materials and Reagents
Equipment
Software
Procedure
Recipes
Acknowledgments
This protocol was modified in part from Krebs et al. (2010) and Halperin et al. (2003). This work was supported by grants from the National Science Foundation (MCB-0343279; IOS-0820112) and the Will W. Lester Endowment, University of California. It is dedicated to Stephen Halperin who tragically and unexpectedly passed away.
References
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