Abstract
Transposable elements (TEs) are a major component of all genomes, thus the epigenetic mechanisms controlling their activity is an important field of study. Cytosine methylation is one of the factors regulating the transcription and transposition of TEs, alongside Histone modifications and small RNAs. Adapter PCR-based methods [such as Amplified Fragment Length Polymorphism (AFLP)] have been successfully used as high-throughput methods to genotype un-sequenced genomes. Here we use methylation-sensitive restriction enzymes, in combination with PCR on adaptor-ligated restriction fragments, to evaluate epigenetic changes in TEs between genomic DNA samples.
Keywords: DNA methylation, Transposable elements, TMD, Transposon methylation display, Cytosine methylation
Materials and Reagents
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Acknowledgments
The transposon methylation display method (TMD) was adapted from the amplified fragment length polymorphism (AFLP) (Vos et al. 1995), and used first by Shaked et al. (2001). This work was supported by a grant from the Israel Science Foundation (grant # 142/08) to Khalil Kashkush.
References
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