Search

Wax Analysis of Stem and Rosette Leaves in Arabidopsis thaliana   

Download PDF How to cite Favorites 1 Q&A Share your feedback Cited by

In this protocol

Original research article

A brief version of this protocol appeared in:
Plant Physiology
Nov 2012

Abstract

The primary aerial surfaces of all land plants are coated by a lipidic cuticle, which restricts non-stomatal water loss and protects the plant from pathogens, herbivores, and ultraviolet radiation. The cuticle is made up of two components: cutin, a polymer of hydroxy- and epoxy- long-chain fatty acid derivatives and glycerol, and cuticular waxes, which are derivatives of very-long-chain fatty acids. While chemical analysis of cutin can be a lengthy and technically challenging task, analysis of cuticular waxes is relatively simple, and can be routinely used to characterize different plant species, adaptations of a given species to environmental conditions, or mutant phenotypes. Here, we present a protocol tailored for the analysis of cuticular waxes on the surface of the model organism Arabidopsis thaliana. Because cuticular waxes are found on the outermost surface of the plant, the wax extraction process is very simple, and sample processing can be completed in less than one day. Chemical analysis involves quantitation of wax monomers by gas chromatography coupled with flame ionization detection (GC/FID), and identification of wax monomers by either mass spectrometry or comparison of retention times of individual wax components to those of known standards.

Keywords: Arabidopsis, Cuticle, Wax, Lipid, Gas chromatography

Copyright: © 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Haslam, T. M. and Kunst, L. (2013). Wax Analysis of Stem and Rosette Leaves in Arabidopsis thaliana. Bio-protocol 3(11): e782. DOI: 10.21769/BioProtoc.782.
  2. Haslam, T. M., Manas-Fernandez, A., Zhao, L. and Kunst, L. (2012). Arabidopsis ECERIFERUM2 is a component of the fatty acid elongation machinery required for fatty acid extension to exceptional lengths. Plant Physiol 160(3): 1164-1174.
Q&A

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.

You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.

girija chalumuru
university of horticultural sciences, karnataka, india
ITS A VERY WORTH-FULL PROTOCOL ABOUT LEAF CUTICULAR WAX. SIR CAN I HAVE A STANDARD PROTOCOL FOR ESTIMATION OF EPICUTICULAR WAX THROUGH CALORIMETRIC METHOD WITH STANDARD CURE OF ANY NATURAL WAX, AS I AM A RESEARCH SCHOLAR CONDUCTING MY RESEARCH ON TUBEROSE WAX ESTIMATION .

PLEASE KINDLY ATTACH THE PROTOCOL TO MY MAIL ID FOR THE SAME IF AVAILABLE.


THANK YOU SIR
11/13/2013 12:25:39 AM Reply