Abstract
Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing cell death activity and disease resistance function of the barley MLA (mildew locus A) disease resistance proteins against the powdery mildew fungus, which can be further adopted for other purposes for other types of plant proteins and in some other plant species, including Arabidopsis thaliana.
Keywords: Transient gene expression, Single epidermal cell, Particle bombardment, DNA coating, Barley, wheat
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from Shirasu et al. (1999); Shen et al. (2012) and Bai et al. (2012).
References
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