A Nucleocapsid-based Transcomplementation Cell Culture System of SARS-CoV-2 to Recapitulate the Complete Viral Life Cycle

Materials and Reagents

1. Materials

   1. Electroporation cuvettes, 4-mm gap (Bio-Rad, catalog number: 1652088)

   2. 24-well plate (Thermo Fisher Scientific, catalog number: 142475)

   3. 96-well plate (Thermo Fisher Scientific, catalog number: 167008)

   4. 0.2-ml PCR tubes (Thermo Fisher Scientific, catalog number: N8010580)

   5. 1.5-ml tube (Corning, catalog number: MCT-150-C)

   6. 15-ml tube (Thermo Fisher Scientific, catalog number: 339650)

   7. 10-cm dish (Thermo Fisher Scientific, catalog number: 150466)




2. Cells

   1. Caco-2 cells (ATCC, catalog number: HTB-37)

   2. 293T cells (ATCC, catalog number: CRL-3216)

   3. Stbl3 competent cells (AlpaLife, catalog number: KTSM110L)

   4. EPI300 competent cells (Lucigen, catalog number: C300C105)




3. Reagents

   1. Dulbecco’s Modified Eagle Medium (DMEM; Gibco, catalog number: C11965500BT)

   2. 0.25% Trypsin-EDTA (Thermo Fisher Scientific, catalog number: 25200072)

   3. Fetal bovine serum (BIOVISTECH, catalog number: SE100-011)

   4. Penicillin/streptomycin, 10,000 U/ml (Thermo Fisher Scientific, catalog number: 15140122)

   5. DNA transfection reagent Vigofect (Vigorous, catalog number: T001)

   6. pMD2G (Addgene, catalog number: 12259)

   7. psPAX2 (Addgene, catalog number: 12260)

   8. Opti-MEM^TM (Gibco, catalog number: 31985070)

   9. 5 kb DNA marker (Takara, catalog number: 3428A)

   10. 15 kb DNA marker (Takara, catalog number: 3582A)

   11. PrimeSTAR^® Max DNA Polymerase (Takara, catalog number: R045)

   12. PrimeSTAR^® GXL DNA Polymerase (Takara, catalog number: R050)

   13. Restriction enzyme BsaI (NEB, catalog number: R0535L)

   14. T4 DNA Ligase (NEB, catalog number: M0202L)

   15. mMESSAGE mMACHINE^TM T7 transcription kit (Thermo Fisher Scientific, catalog number: AM1344)

   16. E.Z.N.A^® Plasmid DNA Mini Kit I (Omega, catalog number: D6943-02)

   17. E.Z.N.A^® Gel Extraction Kit (Omega, catalog number: D2500-02)

   18. Phenol:chloroform:isoamyl alcohol 25:24:1 (pH 7.8; Solarbio, catalog number: P1012)

   19. ReverTra Ace^® qPCR RT Kit (Toyobo, FSQ-101)

   20. TRIzol^TM Reagent (Thermo Fisher Scientific, catalog number: 15596018)

   21. CopyControl^TM Induction Solution (Lucigen, catalog number: CCIS125)

   22. Polybrene (Sigma, catalog number: TR-1003-G)

   23. Dulbecco's Phosphate-Buffered Saline (PBS; Corning, catalog number: 21-031-CVR)

Equipment

1. Water bath (zhybioresources, catalog number: SYG-1210 )

2. Centrifuge (Eppendorf, catalog number: 5406000291)

3. Blue Light Gel Imager (Sangon Biotech, catalog number: G500312)

4. NanoDrop^TM One/Onec (Thermo Fisher Scientific, catalog number: 701-058108)

5. Gene Pulser Xcell Total Electroporation System (Bio-Rad, catalog number: 1652660)

6. Incubator shaker (Changzhou Huayi, catalog number: THZ-D)

Procedure

Timeline:

1. Propagation of plasmids bearing SARS-CoV-2 cDNA fragments

   Steps 1-2, Chemical transformation (2 h) and colony screen (overnight).

   Step 3, Plasmid preparation: 1 h.

2. Construction of Caco-2-N and Caco-2-N^intein cell line

   Steps 1-2, Lentivirus packaging (3) and transduction (3 d).

3. Preparation of DNA fragment by PCR

   Steps 1-3, PCR amplify fragments A-B (6 h), C, D and E (2-3 h).

   Steps 4, Purification of PCR products by phenol-chloroform extraction: 1-2 h.

4. Generation of genome-length cDNA by restriction enzyme digestion and in vitro ligation

   Steps 1-2, Fragments C, D, and E digestion (5 h) and purification (1-2 h).

   Step 3, Fragments C, D, and E in vitro ligation: 24 h.

   Step 4, PCR amplification of C-D-E (6 h) and purification (1-2 h).

   Step 5, Fragments A-B and C-D-E digestion: 5 h.

   Step 6, Full-length cDNA assembly by in vitro ligation: 48 h.

5. Generation of viral genome-length RNA by in vitro transcription (IVT)

   Step 1, PCR amplification of N gene (1 h) and purification (1-2 h).

   Steps 2-8, In vitro transcription: 1 d.

6. Electroporation and trVLP recovery

   Steps 1-4, Electroporation: 2 h.

   Steps 5-10, trVLP recovery: 3-4 d.

7. RT-PCR for verification of GFP gene of the trVLP

   Step 1, trVLP infection (24 h) and viral RNA extraction: 1-2 h.

   Step 2, RT-PCR for amplification of GFP gene (1-2 h) and analysis by agarose electrophoresis (30 min).

8. trVLP titration by TCID₅₀

   Steps 1-5, trVLP infection (24 h) and TCID₅₀ calculation (1-2 h).

9. Propagation of plasmids carrying SARS-CoV-2 cDNA fragments

   1. Plasmids

      The genome of SARS-CoV-2 GFP/ΔN (derived from the Wuhan-Hu-1 strain. GenBank: MN908947 was divided into four fragments designated as A-B, C, D, and E, each obtained by PCR using a chemically synthesized SARS-CoV-2 genome as the template. The fragments were cloned into the pCCI, pMV, or pLVX vectors, resulting in four plasmids: pCCI-A-B, pMV-C, pLVX-D, and pLVX-E. The SARS-CoV-2 N gene with a Flag tag in the N terminal was cloned into the lentivirus vector pLVX-IRES-mCherry, resulting in pLVX-N-Flag-IRES-mCherry that was then was used to generate lentivirus. Caco-2 cells transduced with this lentivirus (Caco-2-N) serve as the packaging cell line for producing SARs-CoV-2 trVLPs, as the Caco-2-N cells complement the SARS-CoV-2 GFP/ΔN genome by providing N in trans. Meanwhile, N gene was also mutated at 212 amino acid (G212C) and split into two parts. These two parts are fused with intein elements Npu-N and Npu-C (Zettler et al., 2009) separately defining as N^N-Int^N and Int^C-N^C and further cloned into lentiviral vectors, resulting pLVX- N^N-Int^N-IRES-mCherry and pLVX-Int^C-N^C-IRES-puromycin, respectively. These two plasmids encoding either N- or C-terminal of N protein were transduced together to ligate the full-length N in Caco-2 cell for generating another packaging cell line Caco-2-N^intein.

   2. Transformation

      1. For each plasmid, thaw EPI 300 or Stbl3 competent cells on ice (see "Note" below) and add 2 μl (approximately 10 ng) of the respective plasmid to the competent cells. Mix by tapping the tube and incubate on ice for 30 min.

         Note: For pMV- and pLVX-derived plasmids – including pMV-C, pLVX-D, pLVX-E, pLVX-N-Flag-IRES-mCherry, pLVX-N^N-Int^N-IRES-mCherry, and pLVX-Int^C-N^C-IRES-puromycin – use Stbl3 competent cells for transformation. For pCCI-derived plasmid pCCI-A-B, EPI300 competent cells for transformation.

      2. Heat shocks the tube containing the cells and plasmid mixture in a 42°C water bath for 90 s, then immediately place back on ice for a 2 min incubation.

      3. Add 1 ml LB medium to the tube and shake at 37°C for 45 min at 220 rpm using an incubator shaker to recover the cells.

      4. After recovery, plate 100 μl of the cell culture onto an LB plate supplemented with the appropriate antibiotics (see "Notes" below) and incubate overnight at 37°C.

         Notes:

         1. For selection of Stbl3 bacteria transformed with pMV- and pLVX-derived plasmids, use LB agar plates supplemented with 50 μg/ml ampicillin.

         2. For selection of EPI300 bacteria transformed with pCCI-A-B, use LB agar plates supplemented with 12.5 μg/ml chloramphenicol.

   3. Preparation of plasmids carrying SARS-CoV-2 genes

      1. After overnight incubation, for each plate, pick 2-3 bacterial colonies and inoculate 4 ml LB supplemented with the appropriate antibiotics (the antibiotic concentration remains the same as on the plates). Incubate overnight (12-16 h) at 37°C with shaking at 220 rpm.

      2. For pMV- and pLVX-derived plasmids, the overnight culture can directly be used for plasmid preparation. For pCCI-A-B, the bacterial culture should be further induced as follows:

         Add 1.5 ml of the overnight culture to a 50 ml flask or tube containing 13.5 ml LB supplemented with 12.5 μg/ml chloramphenicol and 15 μl of CopyControl^TM Induction Solution (Lucigen, catalog number: CCIS125), and shake at 220 rpm for 5 h at 37°C.

      3. Centrifuge the cell cultures by spinning at 4,000 × g for 5 min.

      4. Discard the supernatant and remove as much residual liquid as possible.

      5. Following the manufacturer's instructions, use the cell for plasmid extraction via the Plasmid Mini Kit I (Omega).

      6. At the final step of plasmid extraction, add 50 μl deionized water to the mini-prep column and incubate at RT for 3 min, then centrifuge at 13,000 × g for 1 min to elute the plasmids.

      7. Determine the concentration and quality of the plasmid prep using a NanoDrop. The plasmids can be used immediately or stored at -20°C until use.




10. Construction of Caco-2-N and Caco-2-N^intein cell lines

    1. Lentivirus packaging

       Prepare vesicular stomatitis virus G protein (VSV-G) pseudotyped lentivirus following this published protocol (Tiscornia et al., 2006). Briefly, use VigoFect DNA transfection reagent to transiently co-transfect HEK293T cells with the third-generation packaging plasmids pMD2G (catalog no. 12259; Addgene), psPAX2 (catalog no. 12260; Addgene), and the transfer vector pLVX-N-Flag-IRES-mCherry. Change the medium 12 h post transfection and collect supernatants at 36, 60, and 84 h post transfection. Pool all the supernatants, pass through a 0.45-μm filter, aliquot, and store at -80°C until needed.

    2. Transduction

       1. Seed 1 × 10⁵ Caco-2 cells in a 24-well plate one day prior to infection to ensure 70-80% confluent next day.

       2. When ready for transduction, remove the cell culture medium, add 1 ml of lentivirus supplemented with 10 μg/ml polybrene into the well, and mix by rocking the plate gently. Incubate at 37°C, 5% CO₂ for 48 h.

          Notes:

          1. To generate the Caco-2-N cell line, lentivirus packaging pLVX-N-Flag-IRES-mCherry is used for transduction. To generate the Caco-2-N^intein cell line, lentivirus packaging pLVX-N^N-Int^N-IRES-mCherry and pLVX-Int^C-N^C-IRES-puromycin are mixed (1:1) and used for transduction of the Caco-2 cells.

          2. The addition of polybrene increases transduction efficiency.

       3. As a measure of transduction efficiency, monitor the mCherry signal daily using a fluorescent microscope. Generally, the mCherry signal can be readily detected at 48 h post infection.

       4. At 48 h post transduction, check the mCherry signal and passage the transduced cells into a 6-well plate and incubate at 37°C, 5% CO₂.

       5. At another 24 h, check the mCherry signal and cell density to make sure at least 90% of cells are mCherry positive, harvesting cells that could be used as the packing cell line for production of trVLPs.

       Note: Since these cells are not under selection, the N gene in these Caco-2-derived cell lines can be lost after several passages, which will decrease the packaging efficiency of trVLPs. Thus, re-transduce the Caco-2-N cell lines as needed to maintain the N gene expression level. Alternatively, use a cell sorter to harvest the first 30% of the mCherry-positive cells from the constructed Caco-2-N cell lines if the N expression level is not sufficient (the mCherry positive cells lower than 90%) for trVLP packaging.




11. Generation of SARS-CoV-2 trVLP cDNA by PCR

    1. The template, primers, and DNA polymerase in the thermal cycling program for PCR are listed in Table 1. The sequences of primers used in this protocol are listed in Table 2.

       
       Table 1. Components for PCR reactions to generate select DNA fragments
       

       
       Table 2. Primers used in this protocol
       

       
       

    2. Amplification of fragments A-B

       1. Set up a 50 μl PCR reaction using PrimeSTAR^® GXL following the manufacturer’s instructions. Prepare a 400 μl reaction master mix and aliquot it into eight 0.2-ml PCR tubes (50 μl/tube). The components for the PCR reaction are listed in Table 3.

          
          Table 3. PCR reaction for amplification of fragment A-B
          

          
          

       2. Incubate reactions in a thermal cycler according to the program shown in Table 4.

          
          Table 4.Thermal cycling program for PCR reaction to amplify fragment A-B
          

          Note: Usually, 30-40 μg of fragment A-B can be recovered from the 400 μl PCR reaction solution.

          
          

       3. Examine the PCR products by gel electrophoresis. Mix 2 μl of PCR product and with 6× DNA loading buffer and load the mixture onto a 1% agar gel (Figure 2A).

          
          
          Figure 2. Agarose gel electrophoresis verification of DNA and RNA fragments for trLVPs generation. (A) The genome sequence of trVLPs was divided into four fragments and each fragment PCR amplified. The purified PCR products of each fragment were determined by agarose gel. (B) PCR products of C-D-E and purified ligation products of full-length DNA (FL DNA). (C) Agarose gel analysis of SARS-CoV-2 N gene PCR products. (D) Agarose gel analysis of viral full-length RNA (FL-RNA) and (E) N gene mRNA generated by in vitro transcription using the FL DNA genome and N gene PCR products, respectively, as template. The black arrow indicates the FL RNA and N RNA, respectively.

          
          

    3. Amplification of fragments C, D, and E

       1. Set up a 50 μl PCR reaction according to the PrimeSTAR^® Max (Takara) instructions. Prepare a 200 μl PCR reaction for each fragment and aliquot it into four 0.2 ml PCR tubes, 50 μl/tube. The components for the PCR reaction are listed in Table 5.

          
          Table 5. PCR reaction for amplification of fragments C, D, and E
          

          
          

       2. Incubate reactions in a thermal cycler according to the program shown in Table 6.

          
          Table 6. Thermal cycling program for PCR reaction of fragments C, D, and E
          

          Note: Usually, approximately 20 μg of each fragment can be recovered from the 200 μl PCR reaction solution.

          
          

       3. Check the quality of the PCR products by gel electrophoresis. Mix 2 μl of PCR product and with 6× DNA loading buffer and load the mixture onto a 1% agar gel (Figure 2A).

    4. Purification of PCR products by phenol-chloroform extraction

       1. Pool the PCR products for each fragment into a 1.5-ml tube, add an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1), and mix thoroughly and centrifuge at 13,000 × g for 1 min at 4°C.

       2. Transfer the upper aqueous phase into a fresh 1.5-ml tube, add chloroform equal to the aqueous phase volume, mix thoroughly, and centrifuge at 13,000 × g for 1 min at 4°C.

       3. Pipet the top layer into a new nuclease-free 1.5-ml tube and add 1/10^th volume of 3 M sodium acetate (pH 5.2). Then add isopropanol in a volume ratio of 1:1, mix well and incubate at -20°C for at least 30 min.

       4. Centrifuge at 13,000 × g for 15 min at 4°C to pellet the DNA.

       5. Remove the supernatant, wash the pellet by adding 1 ml 75% ethanol, and centrifuge at 13,000 × g for 5 min at 4°C.

       6. Remove the residual liquid completely, being careful not to remove any pellet.

       7. Air dry the pellet until it is no longer visible and resuspend in 100 μl of nuclease-free water.

       8. Measure the yield and quality of the recovered DNA by using a NanoDrop.

       9. Check the quality of the purified DNA by gel electrophoresis (Figure 2A).




12. Generation of genome-length cDNA by restriction enzyme digestion and in vitro ligation

    Fragments C, D, and E are first digested with Bsa I and ligated by T4 ligase to create the C-D-E ligation product, which is utilized as a template for PCR amplification. Next, the PCR products of fragment A-B and C-D-E are digested with BsaI and ligated to generate the full-length SARS-CoV-2 cDNA.

    1. Fragments C, D, and E digestion

       1. Set up an 80-μl digestion reaction for fragments C, D, and E, as shown in Table 7. Fragments C and E are digested with Bsa I.

          
          Table 7. Fragments C, D, and E digestion reactions
          

          
          

       2. Incubate the fragments C, D, and E digestion reactions at 37°C for 5 h or incubate overnight.

    2. Digested DNA fragment purification from agarose gel

       1. After digestion, add 16 μl 6× DNA loading buffer to each reaction tube, mix thoroughly, and load the mixture onto a 1% agarose gel and run at 180 V for 15 min.

       2. Visualize the DNA fragment using a Blue Light Gel Imager. Avoid using UV light for visualization as it can cause DNA damage that will result in failure of downstream RNA transcription.

       3. Cut the target bands (the sizes of the expected C, D, and E bands are 6,133 bp, 4,168 bp, and 1,636 bp, respectively) and extract the DNA fragment from the gel using a gel extraction kit (Omega) following the manufacturer's instructions.

       4. At the final step, add 30 μl deionized water (pre-warmed at 65°C) onto the column and incubate at 37°C for 5 min.

       5. Centrifuge at 13,000 × g for 1 min to elute the DNA.

       6. Re-load the DNA elution onto the column and repeat Steps D2d and D2e to increase the recovery efficiency.

       7. Measure quantity and quality of the recovered DNA using a NanoDrop.

          Note: The recovery efficiency is approximately 30%.

    3. In vitro ligation of fragments C, D, and E

       1. Set up a 10-μl ligation reaction according to the components listed in Table 8. In this protocol, equal molar concentrations of fragments digested by BsaI are used.

          
          Table 8. Fragments C, D, and E ligation reaction
          

          
          

       2. Incubate the reaction system at 4°C for 24 h. The ligation products (without purification) are utilized as a template for amplifying fragment C-D-E.

    4. PCR amplification and purification of fragment C-D-E

       1. Set up a 50 μl PCR reaction using the ligation products from Step D3b as the template. Prepare a 400 μl reaction and aliquot it across eight 0.2-ml PCR tubes (50 μl/tube). The components are shown in Table 9.

          
          Table 9. PCR reaction for amplification of fragment C-D-E
          

          
          

       2. Incubate reactions in a thermal cycler according to the program shown in Table 10.

          
          Table 10. Thermal cycling program for PCR amplification of fragment C-D-E
          

          
          

       3. Purify the PCR products by phenol-chloroform extraction as described in Step C4.

          Note: Approximately 40 μg of C-D-E fragment can be recovered from the 400 μl PCR reaction solution.

       4. Load 2-μl onto a 1% agarose gel electrophoresis to check the quality of the purified PCR product, as shown in Figure 2B.

    5. Fragments A-B and C-D-E digestion with BsaI

       1. Set up a 120-μl digestion reaction for fragments A-B and C-D-E as shown in Table 11.

          
          Table 11. Digestion reaction for fragments A-B and C-D-E
          

          
          

       2. Incubate the digestion reaction at 37°C for 5 h.

       3. After digestion, the digestion products are separated by agarose electrophoresis and recovered by gel extraction using a kit as described in Step D2.

       4. At the final step of extraction, use 100 μl nuclease-free water for elution.

    6. In vitro ligation of fragments A-B and C-D-E

       1. Set up a 100-μl ligation reaction, using equal molar concentrations of the fragments to assemble the full-length cDNA. The reaction components are listed in Table 12.

          
          Table 12. Fragments A-B and C-D-E ligation reaction
          

          
          

       2. Incubate the reaction at 4°C for 24 h.

       3. Add 1 μl of T4 ligase to the ligation reactions, mix well, and continue incubating at 4°C for another 24 h.

       4. Purify the full-length cDNA ligation products by phenol-chloroform extraction as described in Section C. At the final step of purification, dissolve the DNA pellet in 10 μl nuclease-free water. The purified DNA can be used immediately or stored at -20°C.

       5. Confirm the quality and size of the product by gel electrophoresis, loading 2 μl of purified ligation product onto a 1% agarose gel (Figure 2B).




13. Generation of viral genome-length RNA by in vitro transcription (IVT)

    1. Preparation of N gene DNA.

       In this protocol, N gene mRNA is electroporated into Caco-2-N cells along with genome-length viral RNA to increase trVLP production.

       1. Set up a 50 μl PCR reaction according to the PrimeSTAR^® MAX instructions. The PCR reaction components are shown in Table 13.

          
          Table 13. PCR system for amplification of N gene
          

          
          

       2. Incubate reactions in a thermal cycler following the program in Table 14.

          
          Table 14. Thermal cycling program for PCR amplification of N gene
          

          
          

       3. Check the quality of the PCR products by gel electrophoresis. Mix 2 μl PCR product with 6×DNA loading buffer and load the mixture onto a 1% agarose gel (Figure 2C).

       4. Recover N gene PCR products by phenol-chloroform extraction as described in Step C4. At the final step of purification, dissolve the DNA pellets in 30 μl nuclease-free water.

    2. Set up a 30 μl IVT reaction to generate full-length viral RNA and N gene mRNA using the Thermo mMESSAGE mMACHINE T7 transcription Kit, following the reaction setup shown in Table 15.

       
       Table 15. IVT reaction for generating full-length viral RNA and N gene mRNA
       
       

       
       

    3. Incubate the IVT reaction at 32°C for 5 h.

    4. Add 1 μl DNase to the reaction and incubate at 37°C for 15 min to digest the DNA template.

    5. Recover the viral RNA and N gene mRNA using lithium chloride precipitation following the manufacturer’s instructions in the mMESSAGE mMACHINE^® T7 transcription Kit. At the final step of the precipitation, dissolve the RNA pellet in 30 μl nuclease-free water.

    6. Measure the concentration of RNA using a NanoDrop. Usually, approximately 30 μg of viral RNA can be obtained.

    7. Load 1 μg of RNA onto a 1% agarose gel to determine the quality of the genome-length RNA and N mRNA, as shown in Figures 2D and 2E.

    8. Store the RNA at -80°C or use immediately.




14. Electroporation of the genome-length viral RNA and N gene mRNA into Caco-2-N cell for trVLP production

    1. Seed 3 × 10⁶ Caco-2-N cells in a 10-cm dish 2 days before electroporation to make sure sufficient cells are present for electroporation.

    2. Prepare the following reagents and equipment before electroporation:

       1. Cool the centrifuge to 4°C.

       2. Pre-chill DPBS, Opti-MEM, and 4-mm cuvettes on ice.

       3. Pre-warm a 10-cm dish containing 10 ml cell culture medium in a 37°C cell incubator.

    3. Harvest Caco-2-N cells

       1. Add 2 ml of 0.25% trypsin/EDTA to the Caco-2-N cells in the 10-cm dish and incubate at 37°C for approximately 8 min to detach the cells.

       2. Add 2 ml of cell culture medium to the plate to stop digestion and pipet to generate a single-cell suspension.

    4. Cell pretreatment and electroporation

       1. Use a hemocytometer to determine the number of cells.

       2. Transfer the appropriate volume of cell suspension containing 8 × 10⁶ cells to a 15-ml tube and pellet the cells by centrifugation at 500 × g for 5 min at 4°C.

       3. Discard the supernatant and wash cells by resuspending the pellet in 10 ml pre-chilled DPBS.

       4. Precipitate cells by centrifugation at 500 × g for 5 min at 4°C.

       5. Remove the supernatant and resuspend the pellet in 400 μl Opti-MEM.

       6. Add 20 μg genome-length viral RNA and 10 μg N gene mRNA to the cell suspension, pipetting up and down to mix thoroughly.

       7. Transfer the entire cell mixture into the pre-chilled 4-mm cuvette, put the cuvette into the electroporation chamber, applying a single pulse (270 V at 950 μF) using the GenePulser apparatus (Bio-Rad).

       8. After electroporation, immediately pipet the electroporated cells into the pre-warmed 10 cm dish containing 10 ml cell culture medium. Gently rock the plate and incubate at 37°C, 5% CO₂.

       9. Monitor GFP signal daily using a fluorescent microscope. Generally, the GFP signal can be observed 12 h post electroporation, as shown in Figure 3A.

    5. Prepare Caco-2-N cells for de novo infection

       Seed 1 × 10⁵ Caco-2-N cells into a 24-well plate 24 h before de novo infection with the generated trLVPs, incubating at 37°C, 5% CO₂.

    6. De novo infection

       1. Check the GFP signal of the electroporated cells 24 h post electroporation

       2. Collect 1 ml supernatant (containing trVLPs) from the electroporated cells (this will be considered P0).

       3. Centrifuge the supernatant at 500 × g for 5 min to pellet cell debris.

       4. Make sure the confluency of the Caco-2-N cells in the 24-well plate seeded above in Step F5 is approximately 70-80%. Remove the cell culture medium and add the collected supernatant containing trVLPs into the well, and incubate at 37°C, 5% CO₂.

    7. Monitor the GFP signal daily of the de novo infected cells (Figure 3B). Generally, the signal will be seen across the well 96 h post infection.

    8. From the de novo infected cells, harvest supernatant containing trVLPs 4 days post infection (defined as P1 virus), centrifuge at 500 × g for 5 min at 4°C, and store as 1 ml aliquots at -80°C.

    9. To amplify the virus, add 50 μl of P1 virus onto Caco-2-N cells in a 10-cm dish (the confluence is 70-80%) and incubate at 37°C, 5% CO₂.

    10. Monitor the GFP signal daily and harvest supernatants at 48 h post infection. Centrifuge at 500 × g for 5 min at 4°C. The collected virus is defined as P2 and can be stored as 1 ml aliquots at -80°C until use.

        
        

        
        Figure 3. Fluorescence microscopy analysis of Caco-2-N cells infected with SARS-CoV-2 GFP/ΔN. (A) GFP and bright-field images of Caco-2-N cells at 12 h post electroporation. (B) Supernatants of Caco-2-N cells at 24 h post electroporation were collected and used to infect naive Caco-2-N cells in a 24-well plate. The GFP and bright-field images 24 h and 48 h post infection are shown.

        
        

15. RT-PCR verification of the GFP gene in SARS-CoV-2 GFP/ΔN trVLPs

    To ensure the presence of the GFP gene in the SARS-CoV-2 GFP/ΔN trVLPs, viral RNA is extracted from the Caco-2-N cells infected by trVLPs and analyzed by RT-PCR (Figure 4A). A primer pair flanking the N region of ORF8 and the 3’UTR was designed for this purpose.

    1. trVLP RNA extraction

       1. Prepare Caco-2-N cells in a 24-well plate one day before infection so that the confluency at time of infection is 70-80%.

       2. Infect cells by adding 50 μl trVLPs into the well and incubate at 37°C, 5% CO₂.

       3. 48 h post infection, discard the supernatant from the trLVP-infected cells and add 500 μl of TRIzol reagent into the well, pipetting up and down to make a single-cell suspension.

       4. Transfer the resultant cell lysate into a fresh 1.5 ml tube, add 100 μl chloroform, and vortex the tube or shake it by hand violently. Centrifuge at 13,000 × g for 15 min at 4°C.

       5. Carefully transfer 200 μl of the upper aqueous phase (containing RNA) to a fresh 1.5 ml tube, mix well with 200 μl isopropanol, and incubate at -20°C for 30 min.

       6. Centrifuge at 13,000 × g for 15 min at 4°C and remove the supernatant.

       7. Add 600 μl 75% ethanol to gently wash the pellet and centrifuge at 13,000 × g for 3 min at 4°C.

       8. Remove the supernatant and centrifuge the tube at 13,000 × g for 1 min at 4°C.

       9. Remove the supernatant and open the tube lid to dry the pellet thoroughly.

       10. Add 20 μl RNase-free H₂O to dissolve the RNA sample.

    2. RT-PCR

       1. Use the Toyobo reverse transcription kit and the supplied random primers according to the manufacturer's direction to generate cDNA from the extracted viral RNA. A total of 10 μl cDNA is obtained that can then be used immediately or stored at -20°C until use.

       2. Add 40 μl H₂O to 10 μl cDNA and mix well.

       3. Set up a 50 μl PCR reaction using the cDNA as the template following the setup shown in Table 16.

          
          Table 16. PCR reaction for verification of GFP gene
          

          
          

       4. Incubate reactions in a thermal cycler following the program shown in Table 17.

          
          Table 17. Thermal cycling program for PCR verification of GFP gene
          

          
          

       5. Examine the size of the PCR products by gel electrophoresis. Mix 5 μl PCR product with 6×DNA loading buffer and load the mixture onto a 1% agarose gel (Figure 4B).
          
          

       
       Figure 4. Analysis by RT-PCR of the GFP gene in trLVPs. RT-PCR validation of the GFP gene in SARS-CoV-2 GFP/ΔN trVLPs generated in Caco-2-N cells. The expected DNA size (A) and agarose electrophoresis analysis of the PCR products (B) are shown.

       
       

16. trVLP titration using a tissue-culture infectious dose 50% (TCID₅₀) endpoint dilution assay

    trVLP infectivity is quantified using a TCID₅₀ endpoint method (Lindenbach, 2009).

    1. Seed 1.5 × 10⁴ Caco-2-N cells in 100 μl DMEM supplemented with 10% FBS per well in a 96-well plate one day before infection.

    2. Thaw the trVLPs stored at -80°C and serially dilute 1:10 using DMEM with 10% FBS (12 μl trVLP sample into 108 μl DMEM with 10% FBS). Mix by vortexing for 5 s and remove 12 μl to add to another aliquot of 108 μl DMEM with 10% FBS (1:100 dilution). Repeat the dilution series out to a 108-fold dilution.

    3. Add 100 μl of the diluted virions to each well of the seeded Caco-2-N cells in the 96-well plate on top of the existing media (to make a total of 200 μl per well), with eight replicates per dilution. Gently rock the plate to mix the virions well and incubate at 37°C, 5% CO₂.

    4. Check the GFP signal of each well 24 h post infection and count the number of GFP-positive wells for each concentration.

    5. Calculate theTCID₅₀ following the Reed & Muench method (Reed and Muench, 1938) using the equations described below:

       1. TCID₅₀/ml = (1 (ml)/the volume of original trVLPs (ml)) ×10^ (proportionate distance + Lower dilution).

       2. Proportionate Distance = (Percentage next above 50% - 50%)/(Percentage next above 50% - Percentage next below 50%).

       3. Lower dilution= 10^ (dilution in which position is next above 50%).

Acknowledgments

This work was funded by the National Natural Science Foundation of China (32041005 to QD), the Beijing Municipal Natural Science Foundation (M21001 to QD), and the Start-up Foundation of Tsinghua University (53332101319 to QD). X.J. was funded by the Postdoctoral Science Foundation of China (2021TQ0182) and the Shuimu Tsinghua Scholar Program.

The original research article in which this protocol was used is Ju et al. (2021).

Competing interests

Q.D. and X.J. have filed a patent application on the use of the SARS-CoV-2 transcomplementation system and its use for anti-SARS-CoV-2 drug screening.

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