Abstract
Circulating anti-dsDNA antibody is a hallmark of SLE both in human patients and in many SLE mouse models. This protocol describes how to measure the frequency of plasma cells producing these antibodies in the spleen of lupus-prone mice. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
Keywords: Lupus, Mouse, ELISpots, Autoimmune, Anti-dsDNA
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research, NY, USA. This work was supported by grants from the NY SLE Foundation (RB), Rheuminations, NIH AI082037 and AR 049938-01, NIH (PO1 AI51392 and the Flow Cytometry and Protein Expression and Tetramer Cores of PO1 AI51392).
References
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