Abstract
Airway hyperresponsiveness to methacholine is an important characteristic of asthma. This protocol describes how to measure airway response to methacholine in vigil and unrestraint mouse. The enhanced pause (PenH) is an index of the airway response to increasing doses of methacholine recorded by whole-body barometric plethysmography.
Keywords: Airway responsiveness, Plethysmography, PenH, Respiratory parameters, Mouse
Materials and Reagents
Equipment
Procedure
Recipes
Notes
Acknowledgments
This protocol was adapted from a previously published paper: Reber et al. (2012). FD was supported by the “fond de dotation recherche en santé respiratoire”, call for tenders 2010.
References
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