Before you do any further experiments, make sure to cryo-freeze about 1/4th portion of cells in 10% DMSO with DMEM in liquid nitrogen. Passage the rest of the cells for RNA/protein isolation, in order to validate for stable expression of protein of interest.
Question1: Should I use puromycin again when i will passage ???
Question2? After thawing from nitrogen tank should i seed with puromycin every time when passage?
Please answer
7/7/2022 7:11:58 PM Reply