Abstract
Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice Ragged Stunt Virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH), can suppress jasmonic acid-mediated antiviral defense through the induction of microRNA319 and facilitate virus infection in rice. To verify this, we performed virus inoculation experiments on wild-type rice plants and miR319-TCP21-associated transgenic rice plants through a modified group inoculation method. Here, we presented the detailed procedure of RRSV propagation and infection process on rice plants.
Keywords: Rice ragged stunt virus, RRSV, Brown planthopper, Virus inoculation
Background
Studying mechanism of viral pathogenesis and screening virus-resistant rice varieties have been doing to overcome rice virus diseases and keep food security. In this field, virus inoculation is the essential and reliable method to evaluate the resistance of a transgenic material or a rice variety. Rice Ragged Stunt Virus (RRSV) can transmit rice ragged stunt disease by brown planthopper in a persistent propagative manner in many Asian countries (Ling et al., 1978; Hibino, 1979). Two classical methods, including single seedling inoculation and group inoculation, had been applied to conduct virus inoculation experiments (Zhang et al., 2013). By modifying traditional group inoculation method, we provided a convenient approach which closely resembles the natural infection condition.
Materials and Reagents
Equipment
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Procedure
Data analysis
Notes
Recipes
Acknowledgments
This work was supported by grants to C. Zhang and J. G. Wu from the National Natural Science Foundation of China (Nos. 31722045; 31772128; 31701757 and 31201491); the National Basic Research Program 973 (2014CB138400); and the Natural Science Foundation of Fujian Province of China, Outstanding Young Scientific Research Plan and Excellent Talent Plan in the New Century of Fujian Province (JA3091 and 2014J06011). We also appreciate the previous work related to virus propagation and infection on rice plants conducted by others.
Competing interests
There are no any conflicts of interest or competing interests.
References
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