Published: Vol 2, Iss 18, Sep 20, 2012 DOI: 10.21769/BioProtoc.260 Views: 68134
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Abstract
Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. During angiogenesis, endothelial cells (ECs) undergo activation after binding of angiogenic factors to their receptors, release of proteases to dissolve the basement membrane, migration towards an angiogenic signal, proliferation, and an increase in cell number for new blood vessel formation. Finally, reorganization of ECs forms the three-dimensional vasculature. HUVEC tube-formation assay is one of the simple, but well-established in vitro angiogenesis assays based on the ability of ECs to form three-dimensional capillary-like tubular structures, when cultured on a gel of growth factor-reduced basement membrane extracts. During the assay, ECs differentiate, directionally migrate to align, branch, and form the tubular polygonal networks of blood vessels.
Keywords: HUVECMaterials and Reagents
Equipment
Procedure
Acknowledgments
The protocol was established based on modifications from previous publications: Chan et al. (2011) and Kong et al. (2007).
References
Article Information
Copyright
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Ko, J. M. and Lung, M. L. (2012). In vitro Human Umbilical Vein Endothelial Cells (HUVEC) Tube-formation Assay. Bio-protocol 2(18): e260. DOI: 10.21769/BioProtoc.260.
Category
Cancer Biology > Angiogenesis > Cell biology assays
Cancer Biology > General technique > Cell biology assays
Cell Biology > Cell isolation and culture > 3D cell culture
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