Dear Prof
I am looking for a protocol to be used for homogenization of animal tissues for estimation of antioxidant parameters (SOD,Catalase, Glutathione peroxidase, GSH, MDA etc).
I have been using 50mM phosphate buffer (7.4) containing 0.1 M EDTA for extraction. Is there any other better protocol.
Some people are using RIPA buffer for extraction but I'm doubtful if it affects the activity of antioxidant enzymes.
Please give me a recipe for animal tissue homogenization buffer for estimation of antioxidant enzymes.
Thank you
Best regards
Niti Sharma
1/11/2021 10:46:17 PM Reply