Abstract
Microfluidics chamber is an ideal tool to study local events that occurring in neuronal projections by perfectly compartmentalizing the cell soma from certain branches. It is very well suited for live cell imaging or immunohistochemistry staining. This protocol has been carefully modified in detail to fit the requirement of primary rat hippocampal neuronal cultures. It can also be applied to a more general neuronal culture purpose in microfluidics.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
Alternative reference protocol can be found on the Millipore website related to AXISTM Axon Isolation Devices. The dissection protocol is modified from a protocol developed in Dr. Michael Lin’s lab, Department of Pediatrics and Bioengineering, Stanford University, USA.
References
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