Abstract
This protocol describes the synthesis of peptides for affinity testing and bioconjugate with solid phase peptide synthesizer at a small scale.
Keywords: Solid phase, Peptide, Fmoc, Scaffold protein, Microcleavage
Materials and Reagents
Equipment
Procedure
Representative data
Figure 1. Representative HPLC chromatography of a peptide synthesized with this protocol. Peak at retention time of 11.68 min is the product peak.
Supplement
Recipes
Acknowledgments
This protocol was adapted or modified from previous work (Coin et al. (2007); Miao et al. (2011); Ren et al. (2010)). This work was supported, in part, by the California Breast Cancer Research Program 14IB-0091 and an SNM Pilot Research Grant (ZC).
References
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