Abstract
Neuropathic pain is one of the highly debilitating chronic pain conditions, for which, currently, there is no therapeutic treatment. In order to reveal the underlying mechanism for neuropathic pain, various animal models have been established (Burma et al., 2016). This protocol describes how to prepare spinal nerve injury model (Kim and Chung, 1992; Rigaud et al., 2008; Masuda et al., 2016), one of the most frequently-used and highly reproducible models in which multiple alterations occur both in the peripheral and central nervous system.
Keywords: Neuropathic pain, Nerve injury, Spinal cord, Mouse, Dorsal root ganglion (DRG), Allodynia
Background
Revealing the underlying mechanism of neuropathic pain is necessary to develop effective therapy for its optimal management. Therefore, various animal models for neuropathic pain have been developed. In particularly, rodent models have been frequently used because they are highly reproducible and exhibit pain hypersensitivity that is also observed in patients with neuropathic pain. In this protocol, we describe how to prepare the mouse spinal nerve injury model.
Materials and Reagents
Equipment
Procedure
Ethic Local animal ethics regarding animal housing and animal experiment need to be followed.
Notes
Acknowledgments
This protocol has been developed based on a previously published procedure (Kim and Chung, 1992; Rigaud et al., 2008) with some modifications. T.M. is granted as a JSPS postdoctoral fellowship for research abroad. This work was supported by Grant-in Aid of for Scientific Research (KAKEN 25117013, 15H02522, 16K18885) from JSPS (to K.I., M.T., Y.K.).
References
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