Published: Vol 2, Iss 13, Jul 5, 2012 DOI: 10.21769/BioProtoc.213 Views: 14916
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Abstract
Two-dimensional agarose gel electrophoresis (2D gel) analysis is used extensively as a method to detect origins of replication. Here, I present a simplified method for the isolation of yeast genomic DNA for 2D gel analysis from a small number of yeast cells. This DNA isolation method is simpler and less time consuming than the traditional method that involves CsCl density gradient centrifugation. This method could be modified for 2D gel analysis in other organisms as well.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Zou and Bi (2008) and Wu and Gilbert (1995).
References
Article Information
Copyright
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Zou, Y. (2012). Yeast DNA Replication 2D Gel Protocol. Bio-protocol 2(13): e213. DOI: 10.21769/BioProtoc.213.
Category
Microbiology > Microbial genetics > DNA
Molecular Biology > DNA > DNA extraction
Molecular Biology > DNA > Electrophoresis
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