Abstract
Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in Arabidopsis.
Keywords: Pseudomonas syringae, Arabidopsis thaliana, Plant innate immunity, Pathogenicity
Background
The A. thaliana-Pto DC3000 model pathosystem is widely used to investigate the molecular mechanisms of microbial pathogenesis and plant innate immunity (Ishiga et al., 2012 and 2016; Ishiga and Ichinose, 2016). Although several inoculation methods have been developed to study the interactions in this model system, none of the methods reported to date are similar to those occurring in nature.
Materials and Reagents
Equipment
Procedure
Data analysis
Student’s t-test was used for comparing two averages of bacterial growth, while ANOVA with post-hoc Tukey’s honest significant difference correction for multiple correction was applied when considering more than two averages of bacterial growth.
Recipes
Acknowledgments
This work was supported, in part, by the Program to Disseminate Tenure Tracking System, MEXT, Japan and JST ERATO NOMURA Microbial Community Control Project. This protocol was modified from previous work (Ishiga et al., 2011).
References
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