Abstract
The light/dark transition test (LDT) is one of the most widely used tests to measure anxiety-like behavior in mice. The test is based on the natural aversion of mice to brightly illuminated areas and on their spontaneous exploratory behavior in response to mild stressors, such as novel environment and light. This test is also sensitive to anxiolytic drugs treatment. The test apparatus consists of a box divided into a small (one third) dark chamber and a large (two thirds) brightly illuminated chamber. Mice are placed into the lit compartment and allowed to move freely between the two chambers. The first latency to enter the dark compartment and the total time spent in lit compartment are indices for bright-space anxiety in mice. Transitions are index of activity-exploration, because of habituation over time. LDT is quick and easy to use, without requiring prior training of animals. Here, we present our protocol that has been able to detect both anxiolytic-like (reduced anxiety) and anxiogenic-like (increased anxiety) behavior in mice.
Keywords: Anxiety, Light dark transition test, Behaviour, Mouse
Materials and Reagents
Equipment
Software
Procedure
Representative data
Figure 2. Sample data from light-dark transition test showing anxiolytic-like behavior of TG mice. A. Time spent in the lit compartment; B. Traveled distance in the lit compartment; C. latency time to enter the dark compartment; D. Number of transitions.
Notes
Acknowledgments
The protocol described here has been adapted from a previous study (Serchov et al., 2015), which succeeded in identifying anxiolytic-like behavior for mice. This work was supported by grants from the German Research Council (DFG) (CA 115/5-4) to D.v.C. and K.B., the European Union FP7 program “MoodInflame” to D.v.C. and German Ministry for Research and Education (DMBF) grant e:bio-Modul I -ReelinSys (Project B: 031 6174A) to K.B.
References
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