Abstract
During the last 20 years intestinal mesenchymal cells (IMCs) have emerged as an important cell type that plays a central role in intestinal development and homeostasis, by providing both structural support and growth regulatory elements. IMCs also actively participate in wound healing responses, thus regulating pathologic conditions such as tissue repair, inflammation, fibrosis and carcinogenesis (Powell et al., 2011). We have recently demonstrated that intestinal mesenchymal-specific signals play important in vivo physiological roles in intestinal inflammation and carcinogenesis (Koliaraki et al., 2012; Roulis et al., 2014; Koliaraki et al., 2015). Here we describe the enzymatic method used for the isolation and culture of mesenchymal cells from the adult mouse intestine.
Keywords: Mouse intestine, Mesenchyme, Fibroblasts, Tissue digestion, Collagenase
Materials and Reagents
Equipment
Software
Procedure
Notes
Recipes
Acknowledgments
This protocol was based on a previous protocol used in our lab (Armaka et al., 2008). This work was supported by FP7 Advanced ERC grant MCs-inTEST (Grant Agreement No. 340217) and Innovative Medicines Initiative Joint Undertaking (IMI JU) project BTCure (Grant Agreement No.115142) to GK.
References
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