Abstract
Dendritic spines are the post-synaptic structures that play a central role in excitatory synaptic transmission. Developmental spinogenesis relies on a variety of stimuli such as those derived from cell-cell communication and their downstream signaling. Here, we describe an in vitro assay of dendritic spine retraction using hippocampal slice culture, in which individual neurons are sparsely and brightly labeled by the Supernova method, for the study of molecular mechanisms of spine development.
Keywords: Spine, Retraction assay, EphA, Spinogenesis
Materials and Reagents
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Acknowledgments
This protocol was adopted from Iwata et al. (2015). This work was supported by the JSPS KAKENHI (15H04263, 16K14559) and MEXT KAKENHI (15H01454).
References
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