Abstract
Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for salinity and drought treatments, the soil-culture method in this study was more applicable to studying natural stress conditions.
Keywords: Salinity, Drought, Kenaf (Hibiscus cannabinus L.)
Materials and Reagents
Equipment
Procedure
Data analysis
RNA was isolated and used for selection reliable reference gene (such as ACT, 18S rRNA, EF1α) for Real-Time PCR (RT-qPCR) assay under salinity and drought stress, and this section was stated clearly in our paper (Niu et al., 2015).
Notes
Recipes
Acknowledgments
This work was supported by grants from the 948 project of the Agricultural Department of China (2013-Z70), the National bast fiber germplasm resources project of China (K47NI201A) and National bast fiber research system of China (CARS-19-E06). This protocol was adapted from our recent publications (Niu et al., 2015).
References
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