Abstract
This protocol aims to introduce methods for sacrificing rats by transcardial perfusion and extracting the brain, and introduce methods for staining the rat brain tissue with c-Fos and Arc antibodies. Please note the expression of the proteins is very sensitive to behavioral paradigm that triggers neural activity.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Kim and Thompson (2011).
References
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