Abstract
Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut it into 1 mm long rings. Next, the rings were cultured in growth factor-reduced matrigel polymerized at 37 °C. Angiogenesis was assessed at 7 days by using an inverted microscope platform.
Materials and Reagents
Equipment
Software
Procedure
Note: The whole experiment should be in aseptic conditions including all materials and reagents. All operations should be sterile.
Notes
Recipes
Acknowledgments
We thank Han et al. for technical advice on Aorta Ring Assay method previously published in Angiogenesis 2012. This work was supported by grants from National Natural Science Foundation of China (No.31171418,81320108003,31371498 for J.W., No.81170308,81370247 for X.Y.H., No.81202948 for L.Z., No.81100141 for J.J.), National High-tech R&D 863 Program (No.2013AA020101) Science and Technology Department of Zhejiang province public welfare projects (No.2013C37054), The National Basic Research Program of China (973Program, No.2014CB965100, 2014CB965103), Major science and technology projects of Zhejiang province (2012C13013-3), National Natural Science Foundation of Chian (No.81573641 for LZ), Zhejiang Provincial Natural Science Foundation (No.LY16H280003 for LZ).
References
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Corning Matrigel is a solubilized basement membrane, it contains TGF-beta, epidermal growth factor, fibroblast growth factor, tissue plasminogen activator and so on. The matrigel is not strict solidify in 37℃. It imitate the growth environment in the cell, the tissue could be growth in the matrigel