Abstract
Measurement of auxin transport capacity provides quantitative data on the physiological machinery involved in auxin transport within plants. This technique is easy to perform and gives quick results. Radiolabelled auxin (indole-3-acetic-acid) is fed into the roots of Medicago truncatula via an agar block. The resulting radioactivity from radiolabelled auxin uptake in the roots is measured with a liquid scintillation counter. Here, we describe the measurement of auxin transport capacity around the nodulation susceptible zone in young seedling roots of M. truncatula in response to rhizobia inoculation. Similar assays could be adapted in other plant species and to answer other biological questions.
Keywords: Indole-3-acetic acid, Nodulation, Acropetal auxin transport, Basipetal auxin transport
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Lewis and Muday (2009), van Noorden et al. (2006), Wasson et al. (2006), Plet et al. (2011) and was performed by Ng et al. (2015). This work was supported by an Australian Research Council Future Fellowship awarded to Ulrike Mathesius (FT100100669).
References
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