Abstract
In utero electroporation (IUE) of mouse cerebellar Purkinje cells allows high expression levels of transgenes without toxicity (Nishiyama et al., 2012). This technique is suitable for co-transfection of multiple plasmid genes. Therefore, it is useful to express various sets of genes such as drug-inducible Cre/loxP constructs and CRISPR/Cas9 genome editing constructs (Takeo et al., 2015). Murine Purkinje cells arise from subventricular zone of fourth ventricle at embryonic day (E) 10-12. IUE at E11.5 into fourth ventricle results the most efficient transfection into Purkinje cells.
Keywords: Purkinje cell, In utero electroporation, Cerebellum
Materials and Reagents
Equipment
Procedure
Representative data
Recipes
Acknowledgments
This protocol was adapted from Nishiyama et al. (2012). This work was supported by Japan Society for the Promotion of Science.
References
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