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Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons   

Edited by
Xuecai Ge
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Original research article

A brief version of this protocol appeared in:
Nature
Jun 2015

Abstract

We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015).

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Heinrich, T., Hübner, C. A. and Kurth, I. (2016). Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons. Bio-protocol 6(7): e1785. DOI: 10.21769/BioProtoc.1785.
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