Hi,
very good and detailed protocol, thanks!
but I have two issues:
1. I didn't get the "expected results" as shown in Figure 1, instead, when concentration is low(0-5ug/mL) : pink color, when concentration larger than 100ug/mL, dark blue which is out of the detection limit of my UV-Vis equipment.( see the figure below)
2. the maximum adsorption peak is not at 638nm, is near 650nm
I'll appreciate it very much if you can help me to figure out my issues!
5/30/2018 1:47:07 PM Reply