Abstract
Histone deacetylases (HDACs) catalyzing the removal of acetyl groups from lysine residues of histone and non-histone proteins play vital roles in regulation of gene transcription. In plants, HDACs can be grouped into three families, including RPD3-type, SIR2-type and plant specific HD2-type HDACs. Here we describe a method to determine plant HDAC enzymatic activity. This protocol includes expression, purification and enzymatic activity assay of recombinant plant HDACs expressed in Escherichia coli (E. coli) and Arabidopsis thaliana (A. thaliana).
Keywords: Histone deacetylases, HDACs, Arabidopsis
Materials and Reagents
Equipment
Procedure
Representative data
For representative data, please see the papers of Luo et al. (2015).
Recipes
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31200965) and the China Postdoctoral Science Foundation (2014M562220). The histone deacetylase activity assay was modified from instruction of Biovision HDAC activity colorimetric assay kit.
References
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