Abstract
Extraction of high quality RNA is an essential step for quantitative reverse transcription PCR (qRT-PCR) and microarray analysis. However, it is not easy to extract high quality RNA from fruit materials, which contain high amounts of polysaccharides, lipids and secondary metabolites. Wan and Wilkins (1994) had developed ‘Hot Borate Method’ to isolate high quality RNA. Here, we describe a modified protocol of the ‘Hot Borate Method’ to isolate high quality RNA from grape berry skin for qRT-PCR and microarray analysis (Suzuki et al., 2015a; Suzuki et al., 2015b).
Keywords: RNA extraction, Grape berry skin, Hot Borate Method
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by the Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry from the Bio-oriented Technology Research Advancement Institution (BRAIN) and by Grants-in-Aid for Scientific Research from The Japan Society for the Promotion of Science (JSPS). We thank Dr. Wan and Dr. Wilkins for showing method of ‘Hot Borate Method’. We also thank Dr. Azuma and Mr. Nakao for helping us making of figures and videos.
References
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