Abstract
Strigolactones (SLs) are carotenoid-derived signaling chemicals containing two lactone moieties in their structures and induce seed germination of root parasitic plants, Striga and Orobanche spp. In the rhizosphere, SLs are essential host recognition signals not only for root parasitic plants but also for arbuscular mycorrhizal fungi. In plants, SLs play important roles as plant hormones regulating shoot and root architecture. Plants produce only trace amounts of chemically unstable SLs, which makes it difficult to determine SL contents in plant tissues. Here, we describe how to extract and quantify sorgomol and 5-deoxystrigol, major SLs produced in sorghum roots.
Keywords: Strigolactone, LC-MS/MS, Sorgomol, 5-deoxystrigol, Quantification
Materials and Reagents
Equipment
Procedure
Recipes
Notes
The root content of SLs varies with nitrogen (N) and phosphorus (P) status of sorghum plant. Under N or P deficiency, root contents of sorgomol and 5-deoxystrigol would be approx. 200 and 300 pg/g root FW, respectively. These values may decrease to 1/100 when the plants are subjected to sufficient N and P. Since sorghum produces relatively large amounts of SLs, single plant (seedling) is enough for SL quantification when grown under N or P deficiency. To minimize an individual difference, it is better to perform experiments with 5 to 10 plants in triplicate.
Acknowledgments
This protocol was adapted from previously published studies, Yoneyama et al. (2013), Yoneyama et al. (2015) and Xie et al. (2015). Kaori Yoneyama was supported by a JSPS Fellowship for young scientists and JSPS Restart Postodoctoral Fellowship.
References
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