Abstract
Fluoro-Jade is a fluorescent derivative used for histological staining of degenerating neurons. This technique is simple and sensitive enough to label distal dendrites, axons, axon terminals as well as neuronal bodies. Fluoro-Jade has excitation and emission peak of 480 and 525 nanometer respectively. It can be visualized using a fluorescein/FITC filter. Some reports have demonstrated that Fluoro-Jade can also be useful to detect glial cell death (Anderson et al., 2013; Damjanac et al., 2007).
Keywords: Fluoro-Jade B, Brain staining, Cell death, Neurone
Materials and Reagents
Equipment
Procedure
Representative data
Figure 2. FJB-positive neurons in the mouse cerebral cortex following ischemic stroke
Notes
To ensure reproducibility between protocols, use the same method of tissue preparation.
Recipes
Acknowledgments
This protocol was adapted from Schmued et al. (1997). This work was supported by grants from the Canadian Institutes for Health Research (CIHR) and the Multiple Sclerosis Scientific Research Foundation of Canada.
References
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