Abstract
Liver stem/progenitor cells (LPCs) are defined as bipotential cells differentiating into both hepatocytes and cholangiocytes. For analyzing their differentiation potential, clonal culture has been used for LPCs isolated by a cell sorter. In addition, we can use the culture to assess functions of target genes on differentiation potential of LPCs. This protocol describes the process of cell isolation and colony assay to examine proliferative and differentiation potential of LPCs.
Keywords: Bipotential, Hepatocyte, Cholangiocyte, Liver, Flow cytometry
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
This work was supported by the Ministry of Education, Culture, Sports, Science and Technology, Japan, Grants-in-Aid for Scientific Research (C) for N. Tanimizu (25460271). This protocol was modified from Tanimizu et al. (2014).
References
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