Original research article

The authors used this protocol in:
Jan 2015

Navigate this Article


Product Analysis of Starch Active Enzymes by TLC    

How to cite Favorites 1 Q&A Share your feedback Cited by


Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the reaction product(s) of starch active enzymes, which can contain α-1, 4 linked and α-1, 6 linked glucose. This was first published in Molecular Microbiology (Cockburn et al., 2015). The TLC protocol is an adapted version of that published by Robyt and Mukerjea (Robyt and Mukerjea, 1994). For a summary of the products generated by starch active enzymes see the review by Hii et al. (2012).

Keywords: Thin Layer Chromatography, Starch, Amylase, Maltooligosaccharides

Materials and Reagents

  1. TLC Silica gel 60 F254 20 x 20 cm plates (Note 1) (Merck Millipore Corporation, catalog number: 1.05715.0001 )
  2. Filter paper circles (15 cm diameter) (VWR International, catalog number: 470204-480 )
  3. MilliQ quality water (dH2O)
  4. Acetonitrile (anhydrous) (Sigma-Aldrich, catalog number: 271004 )
  5. Ethyl acetate (anhydrous) (Sigma-Aldrich, catalog number: 270989 )
  6. 2-propanol (Sigma-Aldrich, catalog number: I9516 )
  7. N-(1-naphthyl)ethylenediamine dihydrochloride (Sigma-Aldrich, catalog number: 222488 )
  8. Methanol (anhydrous) (Sigma-Aldrich, catalog number: 322415 )
  9. Sulfuric acid (Fluka, catalog number: 84716 )
    Note: Currently, it is “ Sigma-Aldrich, catalog number: 84716”.
  10. Dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific, catalog number: D128 )
  11. α-D-Glucose (anhydrous) (Sigma-Aldrich, catalog number: 158968 )
  12. Maltose monohydrate (Sigma-Aldrich, catalog number: M9171 )
  13. Maltotriose (Sigma-Aldrich, catalog number: M8378 )
  14. Maltotetraose, DP4 (Sigma-Aldrich, catalog number: 47877 )
  15. Maltopentaose (TCI America, catalog number: M1023 )
  16. Maltohexaose (Sigma-Aldrich, catalog number: M9153 )
  17. Maltoheptaose (Sigma-Aldrich, catalog number: 284017 )
  18. Isomaltose (Sigma-Aldrich, catalog number: I7253 )
  19. D-Panose (Sigma-Aldrich, catalog number: P2407 )
  20. Glycogen from bovine liver (Sigma-Aldrich, catalog number: G0885 )
  21. Amylopectin from potato starch (Sigma-Aldrich, catalog number: A8515 )
  22. Amylose from potato (Sigma-Aldrich, catalog number: A0512 )
  23. Dextran from Leuconostoc spp. (Sigma-Aldrich, catalog number: 31389 )
  24. Pullulan from Aureobasidium pullulans (Sigma-Aldrich, catalog number: P4516 )
  25. Your enzyme of interest and appropriate buffer
  26. Mobile phase (see Recipes)
  27. Staining solution (see Recipes)
  28. Oligosaccharide standards (see Recipes)
  29. Glycogen solution (see Recipes)
  30. Amylopectin solution (see Recipes)
  31. Amylose solution (see Recipes)
  32. Dextran solution (see Recipes)


Note: All equipment needed is shown in Figure 1.

  1. TLC developing chamber (VWR International, catalog number: 21432-739 )
  2. Chromatography sprayer (Note 2) (Sigma-Aldrich, catalog number: Z529729 )
  3. Oven capable of reaching 120 °C (Boekel Scientific, catalog number: 107905 )
  4. (Optional) MilwaukeeTM Dual Temperature Heat Gun (Thermo Fisher Scientific, Fisher Scientific, catalog number: 19-313-598 )
  5. CorningTM ScholarTM PC-170 Hotplate (Thermo Fisher Scientific, Fisher Scientific, catalog number: 13-641-564 )

    Figure 1.
    Equipment needed for this protocol


Enzyme reaction

  1. Prepare 20x concentrated solutions of the enzyme of interest and the appropriate buffer. The concentration of enzyme required will be enzyme and substrate specific, but 1 µg/ml final concentration (20 µg/ml stock) is likely to be a reasonable starting place.
  2. Mix 90 µl of each substrate to be studied with 5 µl of concentrated buffer and pre-incubate at the desired temperature.
  3. Add 5 µl of the concentrated enzyme, mix by pipetting up and down and take a 20 µl sample, immediately freezing or otherwise halting the reaction. Continue to take samples at 10 min, 20 min and 30 min (Note 3), freezing the samples until you are ready to perform TLC.


  1. Add 100 ml of the mobile phase to the TLC developing chamber. Soak 1 or 2 pieces of the filter paper in the mobile phase and stick it to the wall of the chamber (see Note 4). Replace the lid of the chamber and allow 30 min for equilibration of the mobile phase with the atmosphere of the chamber.
  2. With a pencil draw a line 2 cm from the bottom of the TLC plate and include evenly spaced hashmarks (at least 1 cm apart) for each sample to be loaded. These may be labelled below the line in pencil.
  3. Load 1 µl of each standard and sample. Standards may be combined to save space, though at a slight cost to resolution. For less concentrated samples the initial spot can be allowed to dry prior to addition of another 1 µl drop in the same position. This can be done repeatedly as a method for concentrating the sample.
  4. Once the spots have dried place the TLC plate in the developing chamber, immersing the bottom of the plate into the mobile phase at the bottom. The liquid level of the mobile phase should not come above your pencil line. Replace the cover on the chamber.
  5. Incubate until the mobile phase reaches the top of the plate, approximately 3 h for a 20 cm plate.
  6. To separate longer oligosaccharides (>approximately 12 monosaccharide units), remove the plate from the chamber, allow it to dry and then put it back in the chamber to perform a second ascent.

    Video 1. Steps of TLC procedure


  1. Allow the plate to dry and then spray the plate with the staining solution, coating it evenly.
  2. Allow the plate to dry and then place it in the oven or use a heat gun at 120 °C, until spots become clearly visible, approximately 10 min in oven, somewhat less with the heat gun.


  1. Use the standards to determine the identity of the products produced. Note that sugars containing α-1, 6 linkages will run approximately equivalent to an all α-1, 4 linked oligosaccharide one monosaccharide unit longer, for each α-1, 6 linkage present. e.g. isomaltose, runs at approximately the same distance as maltotriose and panose runs at the same distance as maltotetraose (see Figure 2).

Representative data

Figure 2. Sugar standards. Illustration of the run profile for sugar standards. Maltose through maltoheptaose are α-1, 4 linked polymers of glucose from DP2 to DP7. Isomaltose is glucose α-1, 6 linked to glucose, panose is glucose α-1, 6 linked to maltose and glucosyl maltotriose is glucose α-1, 6 linked to maltotriose. Each standard was at 5 mM and 1 µl was loaded onto the plate.


  1. Smaller format plates can be used, particularly if investigating the production of shorter oligosaccharides (maltoheptaose or smaller).
  2. In the absence of a sprayer the plate can be dipped in the staining solution or the staining solution can be poured over the plate, though streaking is more likely to occur.
  3. This procedure is designed to investigate initial reaction products. It is also useful in some cases, particularly with debranching enzymes (hydrolyzing α-1, 6 linkages), to examine the final reaction products. This can be accomplished by using a higher concentration of enzyme and/or a longer incubation time, allowing the reaction to proceed to completion.
  4. Soaking the filter paper and sticking it to the wall of the chamber is optional, but will shorten the time needed for equilibration to occur. This step is not shown in Video 1.
  5. For storage of the polysaccharide solutions pass through a 0.2 µm filter and add sodium azide to 0.02% and store at room temperature (Lichstein and Soule, 1944). For oligosaccharide solutions, store frozen.


  1. Mobile phase
    Mix 85 ml acetonitrile, 20 ml ethyl acetate, 50 ml 2-propanol, 60 ml water
    Requires approximately 100 ml per assay (less with smaller TLC plates and chambers)
  2. Staining solution
    Add 10 ml of sulfuric acid to 190 ml of methanol
    Dissolve 0.6 g of N-(1-naphthyl)ethylenediamine dihydrochloride
  3. Oligosaccharide standards
    Make to 5 mM in water
  4. Glycogen solution
    Dissolve 150 mg in 50 ml of water
  5. Amylopectin solution
    Dissolve 150 mg in 3 ml DMSO
    Slowly add hot water (above 60 °C) with vigorous stirring, bringing the volume up to 50 ml
  6. Amylose solution
    Dissolve 75 mg in 10 ml DMSO
    Slowly add very hot water (above 80 °C) with vigorous stirring, bringing volume up to 50 ml
  7. Dextran solution
    Dissolve 150 mg in 50 ml of water


This work was supported by startup funds to Nicole Koropatkin. The TLC separation protocol was adapted from the work of Robyt and Mukerjea (1994).


  1. Cockburn, D. W., Orlovsky, N. I., Foley, M. H., Kwiatkowski, K. J., Bahr, C. M., Maynard, M., Demeler, B. and Koropatkin, N. M. (2015). Molecular details of a starch utilization pathway in the human gut symbiont Eubacterium rectale. Mol Microbiol 95(2): 209-230.
  2. Hii, S. L., Tan, J. S., Ling, T. C. and Ariff, A. B. (2012). Pullulanase: role in starch hydrolysis and potential industrial applications. Enzyme Res 2012: 921362.
  3. Lichstein, H. C. and Soule, M. H. (1944). Studies of the effect of sodium azide on microbic growth and respiration: I. The action of sodium azide on microbic growth. J Bacteriol 47(3): 221-230.
  4. Robyt, J. F. and Mukerjea, R. (1994). Separation and quantitative determination of nanogram quantities of maltodextrins and isomaltodextrins by thin-layer chromatography. Carbohydr Res (251) 187-202.
Please login or register for free to view full text
Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Cockburn, D. and Koropatkin, N. (2015). Product Analysis of Starch Active Enzymes by TLC. Bio-protocol 5(20): e1621. DOI: 10.21769/BioProtoc.1621.

If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.

If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.

Truc Mai
John Carroll University
Hello, thank you for your protocol. I performed this analysis recently for the first time; the result was not as expected. And because I have never done a TLC before, I hope you could help me point to where I should troubleshoot my procedure. We don't have the TLC Silica gel 60 F254 as listed in the protocol; so I used the two plates we had to compare. On the left was the Polygram Sil G polyester sheets 20x20 cm (Mfr. No. 805013, Matcherey-Nagel), on the right was a Silica gel on TLC Al foils with fluorescence indicator, 60 Angstrom pore size, 10x20 cm (60800-20EA, Sigma Aldrich). I could see some bands of results mostly from the maltose and maltotriose , but it was not very clear. During the procedure, there was some fluctuation in the oven temperature from 110 to 120 degree Celcius because our oven couldn't hold the temperature very well. Also, at the acid staining step, because we did not have a sprayer, I pour out the staining solution on a glass pane and dip the plates completely, then air dried and put to the oven.

The standards from left to right: D+ galactose, D+glucose, D+xylose, D+maltose, Maltose triose, Maltose monohydrate. On the left plate the last one was all sugars, and the right, just water.

It seems that the TLC Al foil plates worked better, but still the results were not very good. Have you seen this before, and could you give me some suggestions? My email is at trucmai@nmsu.edu.

Thank you very much!
7/23/2020 6:40:42 AM Reply
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.