Abstract
This protocol can be used to compare the cell growth rate of yeast under different growth conditions. It involves the serial dilution and spotting of yeast colonies.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol has been modified and adapted in the Espenshade Lab, Johns Hopkins School of Medicine. Funding to support different projects that have used this protocol has come from NIH – National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases, the Pancreatic Cancer Action Network, and the American Heart Association.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
Sorry, I am not sure what do you ask. The 96 plate picture is just for reference. You can use any plate you like.
You can determine if the colony is yeast or bacteria by looking at the color and shape of the colony. Bacteria colony is usually yellowish and the shape is smooth.You can also use a pipette tip to touch the colony, drop it into water and transfer some to a microscope slide. Observe the colony under light microscope. Bacteria is much smaller than yeast. E.Coli is rod shape. Budding yeast has buds.
How to concentrate yeast culture from OD600=0.70 to 1.0? please suggest easiest way. thanks.