Abstract
Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis pollen tubes followed by a sugar composition analysis by gas chromatography mass spectrometry.
Keywords: Pollen tube, Cell wall, Monosaccharide composition, GC-MS
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was initially described in Dumont et al. (2014). This work was supported by the University of Rouen, the region Haute-Normandie, and the “Trans Channel Wallnet” project that has been selected by the INTERREG IVA program France (Channel) – England European cross-border cooperation programme, which is co-financed by the ERDF. The authors also thank the Labex SynOrg (ANR-11-LABX-0029, CRUNCh network, and the European Regional Development Fund (ERDF 31708).
References
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