Abstract
Transient transformation of Artemisia annua does not depend on chromosomal integration of heterologous DNA, and recombinant DNA can be introduced into plant cells via Agrobacterium aided by vacuum. The leaves of 7th and 8th internode from 4-week-old seedlings were chosen as explants, a vacuum system was applied to facilitate agrobacteria into plant cells, the co-cultivation was in the dark at 25 °C for 36-72 h, then GUS or GFP maker genes were used for testing the efficiency of the transformation. The method is used for quick transferring of genes into Artemisia annua (A. annua) by transient transformation.
Keywords: Artemisia annua, Agrobacterium tumefaciens, Transient transformation
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 61173098, No. 61379081).
References
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