Abstract
Stress tolerance is a multigenic trait that depends on the coordinated action of several genes. Various physiological parameters, such as plant height and weight, total yield, chlorophyll content, photosynthesis rate, level of reactive oxygen species (ROS) and anti-oxidant activity could be correlated directly with the level of stress tolerance potential of any particular genotype. To evaluate the stress tolerance potential of a plant, leaf disc stress tolerance assay is a very rapid and widely acceptable experiment with minimum instrumentation facilities.
Keywords: Leaf disc, Abiotic stress tolerance, Plant, Tobacco, Rapid
Materials and Reagents
Equipment
Procedure
Representative data
Figure 1. Response of tobacco leaf discs in presence of 200 mM NaCl for 4 days. Leaf discs are floated onto water to serve as experimental control and 200 mM NaCl for salinity stress. There is no change in the ‘greenness’ of discs under control condition, while discs are bleached and turned into yellow in the presence of 200 mM NaCl.
Notes
Recipes
Acknowledgments
Authors thank International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India for funding research.
References
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