Abstract
The production of 'reactive oxygen species' (ROS), also termed oxidative burst, is a typical cellular response of animals and plants to diverse biotic and abiotic stresses. Here, we outline the detection of the ROS-burst in plant leaf pieces using a luminol-based bioassay which allows for the detection of chemiluminescence. The assay was originally described by Keppler et al. (1989) and subsequently adapted for other plant cells and tissues (Felix et al., 1999) and also used in recent publications (Albert et al., 2013; Albert et al., 2010; Butenko et al., 2014; Halter et al., 2014). In this protocol we outline a standardized version of this assay including remarks and recommendations for data evaluation and interpretation of results.
Materials and Reagents
Equipment
Procedure
Representative data
Recipes
Acknowledgments
R.A.’s work was supported by Grant 348256/F20 from the Research Council of Norway; and Grant 216856 from the Research Council of Norway and the Deutscher Akademischer Austausch Dienst. M. A. was supported by the Deutsch Forschungsgemeinschaft (AL1426/1-1). The method was recently applied in Butenko et al. (2014).
References
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This has to be compared individually!RLU (relative light units) are not properly defined and vary depending on the individual machine. Thus, one have to set up a few wells in each individual experiment without leaf discs.
Wwhat is the reading(range in RLU) when u add just luminol (as a blank i.e, without any sample) in chemiluminescence assay.