Abstract
This protocol has been designed in order to facilitate the isolation and extraction of total RNA from microspores collected from lily anther sacs. This protocol allows the extraction of high amounts of high quality RNA, as observed in agarose gels.
Keywords: Microspore separation, RNA extraction, Lily plant
Materials and Reagents
Equipment
Procedure
Representative data
Figure 1. Total RNA (20 μg) was isolated from microspores isolated from lily flower buds with different lengths. 1. 34-36 mm buds; 2. 44-46 mm buds, both are at the microspore stage and 3. 60-65 mm buds at the transition between microspore and mature pollen. Total RNA was denatured, fractionated on a formaldehyde-agarose gel and stained with ethidium bromide.
Recipes
Notes
Acknowledgments
The protocol is an adaption from the protocol of Verwoerd et al. (1989). We greatly appreciate their original contribution.
References
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