I have problms with the elution of the antibodies. I checked with a second antibody if those from the serum are still on the menbrane and they are still there. All of them. I don't get a signal on WB with the eluate, and BCA neither shows higher concentration of protein in comparison to elution buffer with Tris. I variated the elution temperature (0- 25 °C) but I am still gtting the same result...
Do you have a solution for my problem?
7/12/2011 5:16:57 PM Reply