Abstract
Plastoglobules are lipoprotein particles physically attached to thylakoids in chloroplasts (Kessler et al., 1999). They are mainly composed of polar lipid, neutral lipids, and proteins (Vidi et al., 2006). Here we used simple sucrose gradient flotation centrifugation method to purify the plastoglobules from total chloroplast membranes (Vidi et al., 2007, Shanmugabalaji et al., 2013).
Keywords: Plastoglobule, Gradient, Chloroplast, Membranes, Centrifugation
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Shanmugabalaji et al. 2013. The project was supported by the NRP59, the University of Neuchatel, NCCR ‘‘Plant Survival’’ (National Center of Competence in Research), SystemsX PGCE and the Swiss National Foundation.
References
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