Abstract
A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to be one of the hallmarks of tumor formation. Here we use the karyotype analysis to separate the chromosomes and observe the chromosomes in tumor cells with a microscope.
Keywords: Chromosome, Chromosomal instability, Tumor formation
Materials and Reagents
Equipment
Procedure
Day 1
Day 3
Representative data
Figure 1. Representative pictures of karyotype analysis in FaDu cell line (see Chou et al., 2013)
Recipes
Acknowledgments
This protocol has been adapted from Chou et al. (2013).
References
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