Abstract
This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes.
Keywords: Meiocyte, Cell cycle, Centromere, Reproduction, Higher plants
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
This work was supported by research grants from Academia Sinica (Taiwan), the National Science and Technology Program for Agricultural Biotechnology (NSTP/AB, 098S0030055-AA), Taiwan, and the National Science Council (NSF; 99-2321-B-001-036-MY3), Taiwan, to G.-Y. Jauh; an NSF grant (MCB0718191) to C. A. Makaroff, and a study-abroad contract from the University of Costa Rica to P. Bolaños-Villegas.
References
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