Abstract
This protocol will result in the accurate qualitative measurement of anti-Chikungunya virus antibody (Ab) from infected mouse tissue or serum. This assay was developed by Dr. Caitlin Briggs, Arbovax, Inc. Chikungunya is a BL3 agent and should be handled in a biosafety level 3 laboratory under BL3 conditions. This protocol was used in the publication “Chikungunya virus host range E2 transmembrane deletion mutants induce protective immunity against challenge in C57BL/6J mice” (Piper et al., 2013).
Materials and Reagents
Equipment
Procedure
All of procedures A and B are performed at the BL3 level unless otherwise specified. Therefore every step involving live intact virus in unsealed containers must be completed inside a laminar flow biosafety cabinet, and the following preparative protocols should be observed before and after work is performed.
Recipes
Acknowledgments
The protocol described here was used in the publication Piper et al. (2013).
References
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